Pre-implantation Genetic Screening using BoBs test is an efficient and rapid method of detecting aneuploidies in all 24 chromosomes. It can also be used to detect copy number changes in DNA extracted from amniotic fluids, CVs and Product of Conception.
KaryoLite™ BoBs™ is a new member of the BACs-on-Beads™ family of products KaryoLite BoBs has been developed to detect aneuploidies, gains and losses in all 24 chromosomes in a single assay. This assay is being offered for research use only. The product covers both p and q arms of all chromosomes 1-22, X and Y. It is a bead-based molecular karyotyping assay utilizing probes derived from BAC DNA and coupled to encoded microspheres. It consists of 90 DNA probes immobilized onto polystyrene microspheres distinguishable by the Luminex® instrument system.
The kit is easy to use with objective and fast result interpretation. The protocol is very robust, assuring results that can be relied on. The kit contains sample labeling reagents, the BACs-on-Beads mix, hybridization reagents, streptavidin-phycoerythrin reporter and wash reagents.
Research may be performed with KaryoLite BoBs using minute amounts of genomic DNA extracted directly from amniotic fluid, chorionic villae or fetal tissue. In fact only 50 ng of DNA is needed, and no cell culturing is required.
For True Rapid Aneuploidy
- Results in less than 24 hours Complete procedure from sample to result takes less than 24 hours, allowing your laboratory to obtain results the following day.
- Quick and easy implementation The KaryoLite BoBs reagents are provided in a single kit.
- Cost-efficiency Tens of samples can be run simultaneously reducing the hands-on time required.
- Easy interpretation Intuitive BoBsoft™ analysis software presents results that are clear and easy to interpret.
- More information Aneuploidies, gains and losses in all 24 chromosomes in one assay.
- Results are obtained in less than 24 hours and the hands-on-time is only about 3.5 hours.
- The probes used have been carefully selected to provide information about the whole chromosome including the subtelomere region of the chromosome.
- Separate bead types provide coverage of at least two separate regions of each arm of the chromosome.
- In acrocentric chromosomes (13, 14, 15, 21 and 22) three beads cover the q arm of the chromosome.